FastGene® DNA polymerase is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. The enzyme is purified using three different chromatography technologies and result in a very high purity and very high activity. 5U/µL FastGene® DNA Polymerase contain 150ng/µL (±10%) protein of ﹥98% purity. PCR products generated with FastGene® DNA polymerase are A-tailed and therefore compatible with TA cloning systems.
Comes with 2 different reaction buffers. Buffer A is a “high yield” buffer; for most amplicons, when you compare buffer B and buffer A, buffer A will give higher yield. Buffer B is a standard KCl-based Taq buffer which is supplied in case a customer wants to change only the enzyme, and keep the buffer the same. Furthermore a higher sensitivity has been seen by using buffer B in some PCR assays. This is just a guideline, for some applications the reverse will be true.
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